Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'
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چکیده
منابع مشابه
Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'.
Although it has been recognized that PCR amplification of mixed templates may generate sequence artifacts, the mechanisms of their formation, frequency and potential elimination have not been fully elucidated. Here evidence is presented for heteroduplexes as a major source of artifacts in mixed-template PCR. Nearly equal proportions of homoduplexes and heteroduplexes were observed after co-ampl...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2002
ISSN: 1362-4962
DOI: 10.1093/nar/30.9.2083